Ccl2^tm1.1Tyos
Targeted Allele Detail

Summary

• Symbol: Ccl2^tm1.1Tyos
• Name: C-C motif chemokine ligand 2; targeted mutation 1.1, Teizo Yoshimura
• MGI ID: MGI:4355946
• Synonyms: MCP-1^delta
• Gene: Ccl2 Location: Chr11:81926403-81928278 bp, + strand Genetic Position: Chr11, 49.82 cM, cytoband C-E1
• Alliance: Ccl2^tm1.1Tyos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151874
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Mice containing the floxed Ccl2 allele were crossed with EIIa-Cre mice to obtain mice lacking exons 1 and 2. Correct targeting was confirmed by Southern blotting. Supernatant analysis of activated homozygote macrophages did not detect protein product confirming this is a null allele. Similar ELISA analysis demonstrated an increase in MCP-3 secretion, which is encoded by the nearby Ccl7 gene. (J:151874)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ccl2 Mutation: 25 strains or lines available

References

• Original: J:151874 Takahashi M, et al., Monocyte chemoattractant protein-1 (MCP-1), not MCP-3, is the primary chemokine required for monocyte recruitment in mouse peritonitis induced with thioglycollate or zymosan A. J Immunol. 2009 Sep 1;183(5):3463-71
• All: 5 reference(s)