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Rpl22tm1.1Psam
Targeted Allele Detail
Summary
Symbol: Rpl22tm1.1Psam
Name: ribosomal protein L22; targeted mutation 1.1, Paul S Amieux
MGI ID: MGI:4355967
Synonyms: RiboTag, Rpl22HA
Gene: Rpl22  Location: Chr4:152410199-152418528 bp, + strand  Genetic Position: Chr4, 83.03 cM, cytoband E2
Alliance: Rpl22tm1.1Psam page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:151962
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Conditional ready, Epitope tag, No functional change)
Mutation:    Insertion
 
Mutation detailsA loxP site was inserted upstream of the final coding exon (exon 4) and a loxP site and a frt-flanked neomycin cassette were inserted downstream of the exon. In addition, a modified version of exon 4 was knocked-in downstream of the neo cassette. This duplicated exon 4 contained 3 sequence repeats encoding hemagglutinin eptiope (HA) just upstream of the endogenous stop codon. Founder mice were crossed with FlpeR deleter mice to remove the neomycin cassette leaving exon 4 floxed. In the absence of cre recombinase, the stop codon in the endogenous exon 4 prevents translation of the knocked-in exon and only wild-type protein is expressed. In the presence of cre recombinase, the endogenous exon 4 is removed and the modified exon 4 is instead transcribed leading to a ribosomal complex tagged with HA-epitope. (J:151962)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Rpl22 Mutation:  46 strains or lines available
References
Original:  J:151962 Sanz E, et al., Cell-type-specific isolation of ribosome-associated mRNA from complex tissues. Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13939-44
All:  224 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory