Rpl22^tm1.1Psam
Targeted Allele Detail

Summary

• Symbol: Rpl22^tm1.1Psam
• Name: ribosomal protein L22; targeted mutation 1.1, Paul S Amieux
• MGI ID: MGI:4355967
• Synonyms: RiboTag, Rpl22^HA
• Gene: Rpl22 Location: Chr4:152410199-152418528 bp, + strand Genetic Position: Chr4, 83.03 cM, cytoband E2
• Alliance: Rpl22^tm1.1Psam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151962
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of the final coding exon (exon 4) and a loxP site and a frt-flanked neomycin cassette were inserted downstream of the exon. In addition, a modified version of exon 4 was knocked-in downstream of the neo cassette. This duplicated exon 4 contained 3 sequence repeats encoding hemagglutinin eptiope (HA) just upstream of the endogenous stop codon. Founder mice were crossed with FlpeR deleter mice to remove the neomycin cassette leaving exon 4 floxed. In the absence of cre recombinase, the stop codon in the endogenous exon 4 prevents translation of the knocked-in exon and only wild-type protein is expressed. In the presence of cre recombinase, the endogenous exon 4 is removed and the modified exon 4 is instead transcribed leading to a ribosomal complex tagged with HA-epitope. (J:151962)

Expression

In Mice Carrying this Mutation:

1 assay results 11 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rpl22 Mutation: 46 strains or lines available

References

• Original: J:151962 Sanz E, et al., Cell-type-specific isolation of ribosome-associated mRNA from complex tissues. Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13939-44
• All: 232 reference(s)