Rpl22tm1.1Psam
Targeted Allele Detail
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Symbol: |
Rpl22tm1.1Psam |
Name: |
ribosomal protein L22; targeted mutation 1.1, Paul S Amieux |
MGI ID: |
MGI:4355967 |
Synonyms: |
RiboTag, Rpl22HA |
Gene: |
Rpl22 Location: Chr4:152410199-152418528 bp, + strand Genetic Position: Chr4, 83.03 cM, cytoband E2
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Alliance: |
Rpl22tm1.1Psam page
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Allele Type: |
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Targeted (Conditional ready, Epitope tag, No functional change) |
Mutation: |
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Insertion
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Mutation details: A loxP site was inserted upstream of the final coding exon (exon 4) and a loxP site and a frt-flanked neomycin cassette were inserted downstream of the exon. In addition, a modified version of exon 4 was knocked-in downstream of the neo cassette. This duplicated exon 4 contained 3 sequence repeats encoding hemagglutinin eptiope (HA) just upstream of the endogenous stop codon. Founder mice were crossed with FlpeR deleter mice to remove the neomycin cassette leaving exon 4 floxed. In the absence of cre recombinase, the stop codon in the endogenous exon 4 prevents translation of the knocked-in exon and only wild-type protein is expressed. In the presence of cre recombinase, the endogenous exon 4 is removed and the modified exon 4 is instead transcribed leading to a ribosomal complex tagged with HA-epitope.
(J:151962)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:151962 Sanz E, et al., Cell-type-specific isolation of ribosome-associated mRNA from complex tissues. Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13939-44 |
All: |
224 reference(s) |
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