Ifnb1^tm1Lky
Targeted Allele Detail

Summary

• Symbol: Ifnb1^tm1Lky
• Name: interferon beta 1, fibroblast; targeted mutation 1, Richard M Locksley
• MGI ID: MGI:4357661
• Synonyms: Ifnb1^tm1(EYFP)Lky, Ifnb^mob, mob
• Gene: Ifnb1 Location: Chr4:88440262-88441011 bp, - strand Genetic Position: Chr4, 41.91 cM
• Alliance: Ifnb1^tm1Lky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152138
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S/Sv

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A bicistronic reporter cassette containing a loxP-flanked neomycin cassette, an encephalomyocarditis virus (EMCV) IRES element, and enhanced yellow fluorescent protein (EYFP) was introduced immediately after the Ifnb1 STOP codon. The use of 129 background embryonic stem (ES) cells expressing cre under the control of the germline-specific protamine (Prm) promoter allowed for the cre-mediated excision of the floxed neomycin gene from the male germline. The line was backcrossed to C57BL/6 to breed cre out of the line. (J:152138)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ifnb1 Mutation: 21 strains or lines available

Notes

129S/Sv-derived ES cells containing a protamine-cre transgene were used.

References

• Original: J:152138 Scheu S, et al., Visualization of IFNbeta production by plasmacytoid versus conventional dendritic cells under specific stimulation conditions in vivo. Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20416-21
• All: 34 reference(s)