Cyp51^tm1Bfro
Targeted Allele Detail

Summary

• Symbol: Cyp51^tm1Bfro
• Name: cytochrome P450, family 51; targeted mutation 1, Simon Horvat
• MGI ID: MGI:4357830
• Synonyms: Cyp51^flox
• Gene: Cyp51 Location: Chr5:4131145-4154746 bp, - strand Genetic Position: Chr5, 2.3 cM, cytoband A2
• Alliance: Cyp51^tm1Bfro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173366
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: Homology arms were isolated from BAC clone 519E21 (Research Genetics). An frt flanked PGKneo cassette was inserted in intron 3 and loxP sites were inserted in introns 2 and 4. The neo selection cassette was removed from correctly targeted ES cells by Flp mediated recombinase. (J:173366)

Generation of the Cyp51^tm1Bfro and Cyp51^tm1.1Bfro alleles

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyp51 Mutation: 24 strains or lines available

References

• Original: J:173366 Keber R, et al., Mouse knockout of the cholesterogenic cytochrome P450 lanosterol 14alpha-demethylase (Cyp51) resembles Antley-Bixler syndrome. J Biol Chem. 2011 Aug 19;286(33):29086-97
• All: 7 reference(s)