Pax1^{tm1(sb-pLHED)Jtak}
Targeted Allele Detail

Summary

• Symbol: Pax1^{tm1(sb-pLHED)Jtak}
• Name: paired box 1; targeted mutation 1, Junji Takeda
• MGI ID: MGI:4358156
• Synonyms: J0
• Gene: Pax1 Location: Chr2:147203850-147216972 bp, + strand Genetic Position: Chr2, 72.63 cM
• Alliance: Pax1^{tm1(sb-pLHED)Jtak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151776
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A local hopping enhancer detector (LHED) construct was inserted into the 5' flank of the gene, over 49kb upstream of the translation start site. The LHED construct consisted of an frt site, a floxed Pgk1-neo cassette, a puromycin resistance gene, the sleeping beauty transposon pT2, a Pgk1 promoter, and a final frt site. The sleeping beauty transposon pT2 had been modified to contain a loxP site and the mouse Hspa1b minimal promoter fused to a lacZ gene. Removal of the transposon sequence results in expression of the puromycin resistance gene. Local hopping of the transposon allows for the creation of a nested series of deletion/inversion mutations via cre mediated recombination. (J:151776)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pax1 Mutation: 28 strains or lines available

References

• Original: J:151776 Kokubu C, et al., A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in mice. Nat Genet. 2009 Aug;41(8):946-52
• All: 1 reference(s)