Runx1^tm3.1Spe
Targeted Allele Detail

Summary

• Symbol: Runx1^tm3.1Spe
• Name: runt related transcription factor 1; targeted mutation 3.1, Nancy A Speck
• MGI ID: MGI:4358510
• Synonyms: Runx1^f no neo
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm3.1Spe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152374
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Intragenic deletion
• Mutation details: A loxP site flanked targeting vector containing PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Chimeras were crossed to C57BL/6NTac mice for 3 generations then made homozygous. The mice were then crossed to (B6129S)F1/J with the resulting F2 intercrossed to generate homozygotes carrying the floxed allele. These mice were then crossed to transgenic mice expressing Cre recombinase (carrying the Tg(Cdh5-cre)1Spe, (VEC-cre) transgene on a mixed B6;129 background. (J:90431)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:152374 Speck NA, Generation of a Runx1 floxed allele. MGI Direct Data Submission. 2009
• All: 11 reference(s)