Sirt1^tm3Fwa
Targeted Allele Detail

Summary

• Symbol: Sirt1^tm3Fwa
• Name: sirtuin 1; targeted mutation 3, Frederick W Alt
• MGI ID: MGI:4359275
• Synonyms: Sirt1-E4^fl, SIRT1^F, Sirt1^loxP
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^tm3Fwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85542
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed neomycin resistance cassette in reverse orientation was placed at the 3' end of intron 3. An additional loxP site was inserted just downstream of exon 4. In the resulting ES cells, exon 4 is flanked by loxP sites. These mutant ES cells were used in the generation of Sirt1^tm1Fwa mice. (J:85542)

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 81 strains or lines available

References

• Original: J:85542 Cheng HL, et al., Developmental defects and p53 hyperacetylation in Sir2 homolog (SIRT1)-deficient mice. Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9
• All: 58 reference(s)