Mir208a^tm1.1Dzw
Targeted Allele Detail

Summary

• Symbol: Mir208a^tm1.1Dzw
• Name: microRNA 208a; targeted mutation 1.1, Da-Zhi Wang
• MGI ID: MGI:4359822
• Gene: Mir208a Location: Chr14:55186517-55186599 bp, - strand Genetic Position: Chr14, 28.0 cM
• Alliance: Mir208a^tm1.1Dzw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152696
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout) Mutation: Intragenic deletion Mutation details: A DNA fragment from an intron of the Myh6 gene containing the micro RNA coding sequence was replaced with a floxed neo cassette via homologous recombination. Cre mediated recombination then removed the neo cassette leaving a single loxP site in the intron. PCR analysis confirmed the splice pattern of Myh6 was not disturbed. Northern blot analysis confirmed the absence of micro RNA expression in hearts from homozygous mice. (J:152696)

Generation of the Mir208a^tm1Dzw and Mir208a^tm1.1Dzw alleles

Expression

In Mice Carrying this Mutation:

44 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir208a Mutation: 2 strains or lines available

References

• Original: J:152696 Callis TE, et al., MicroRNA-208a is a regulator of cardiac hypertrophy and conduction in mice. J Clin Invest. 2009 Sep;119(9):2772-86
• All: 1 reference(s)