Mirc32^tm1Thbr
Targeted Allele Detail

Summary

• Symbol: Mirc32^tm1Thbr
• Name: microRNA cluster 32, including Mir143 and Mir145a; targeted mutation 1, Thomas Braun
• MGI ID: MGI:4359903
• Synonyms: miR-143/145^-
• Gene: Mirc32 Location: Chr18:61780896-61782329 bp Genetic Position: Chr18, Syntenic

Mirc32^tm1Thbr/Mirc32^tm1Thbr mice develop neointimal lesions

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152702
• Parent Cell Line: MPI-II (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intergenic deletion Mutation details: A 1.3-kb genomic segment containing both microRNAs Mir145 and Mir143 was replaced by a construct containing a lacZ reporter and a neomycin resistance cassette. Inactivation of the loci was confirmed by Northern blot analysis of homozygote aorta, bladder, liver and kidney. Reporter gene expression was detected in early embryonic heart development of heterozygotes, and in most smooth muscle cells of the adult heterozygote. (J:152702)

Generation of the Mirc32^tm1Thbr allele

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc32 Mutation: 3 strains or lines available

References

• Original: J:152702 Boettger T, et al., Acquisition of the contractile phenotype by murine arterial smooth muscle cells depends on the Mir143/145 gene cluster. J Clin Invest. 2009 Sep;119(9):2634-47
• All: 7 reference(s)