Prom1^{tm1.1(DTA)Toko}
Targeted Allele Detail

Summary

• Symbol: Prom1^{tm1.1(DTA)Toko}
• Name: prominin 1; targeted mutation 1.1, Toru Kondo
• MGI ID: MGI:4360366
• Synonyms: Prom1^lacZ,DTA, Prom1^tm1Toko
• Gene: Prom1 Location: Chr5:44150962-44259374 bp, - strand Genetic Position: Chr5, 23.97 cM
• Alliance: Prom1^{tm1.1(DTA)Toko} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152390
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: Exon 2 was replaced with a floxed lacZ gene with a polyadenylation sequence, diphtheria toxin A (DTA) gene, and frt flanked neo cassette. Germ-line flp-mediated recombination removed the neo cassette leaving the floxed lacZ-polyadenylation sequence upstream of the DTA gene. Cre mediated recombination is necessary to achieve expression of the DTA gene. (J:152390)

Generation of the Prom1^tm1.1(DTA)Toko allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prom1 Mutation: 76 strains or lines available

References

• Original: J:152390 Nishide K, et al., Glioblastoma formation from cell population depleted of Prominin1-expressing cells. PLoS One. 2009;4(8):e6869
• All: 4 reference(s)