Ankrd17^tm1.1Yty
Targeted Allele Detail

Summary

• Symbol: Ankrd17^tm1.1Yty
• Name: ankyrin repeat domain 17; targeted mutation 1.1, Yu-Ting Yan
• MGI ID: MGI:4360512
• Synonyms: Ankrd17^delta, Ankrd17^deltaE8
• Gene: Ankrd17 Location: Chr5:90375025-90514436 bp, - strand Genetic Position: Chr5, 44.32 cM, cytoband E2
• Alliance: Ankrd17^tm1.1Yty page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152840
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A floxed neo cassette was inserted upstream of exon 8 and an additional loxP site was inserted downstream of exon 8 via homologous recombination. Cre mediated recombination then removed the neo cassette and exon 8. Removal of exon 8 results in a frame shift creating a premature stop codon at nucleotide 45 in exon 9. Western blot analysis confirmed the absence of protein expression in homozygous embryos. (J:152840)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ankrd17 Mutation: 157 strains or lines available

References

• Original: J:152840 Hou SC, et al., Ankrd17, an ubiquitously expressed ankyrin factor, is essential for the vascular integrity during embryogenesis. FEBS Lett. 2009 Sep 3;583(17):2765-71
• All: 2 reference(s)