Gabra1^tm4Geh
Targeted Allele Detail

Summary

• Symbol: Gabra1^tm4Geh
• Name: gamma-aminobutyric acid type A receptor subunit alpha 1; targeted mutation 4, Gregg E Homanics
• MGI ID: MGI:4361392
• Synonyms: HA
• Gene: Gabra1 Location: Chr11:42021766-42073757 bp, - strand Genetic Position: Chr11, 24.97 cM
• Alliance: Gabra1^tm4Geh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144692
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector was designed (by site-directed mutagenesis) to substitute a serine to histidine at amino acid position 270 and leucine to alanine at amino acid position 277 of exon 9. An FRT-flanked neomycin cassette was inserted upstream of exon 9. Chimeric animals were crossed to C57BL/6J and the F1 progeny crossed to B6.Cg-Tg(ACTFLPe)9205Dym/J (actin-FLPe) to remove the neo cassette and leave a single FRT site upstream of exon 9. (J:144692)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabra1 Mutation: 48 strains or lines available

References

• Original: J:144692 Borghese CM, et al., An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice. J Pharmacol Exp Ther. 2006 Oct;319(1):208-18
• All: 3 reference(s)