Ins2^tm1Mnt
Targeted Allele Detail

Summary

• Symbol: Ins2^tm1Mnt
• Name: insulin II; targeted mutation 1, Massimo Trucco
• MGI ID: MGI:4361633
• Synonyms: Ins2^l
• Gene: Ins2 Location: Chr7:142232393-142233463 bp, - strand Genetic Position: Chr7, 88.0 cM
• Alliance: Ins2^tm1Mnt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152798
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The targeting construct contains loxP sites flanking the mouse Ins2 gene. An Frt-flanked Neo cassette was inserted after the Ins2 exon 3 before the loxP site. The construct was injected into ES cells to modify the endogenous locus through homologous recombination. Mice expressing this allele were generated and subsequently crossed to 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP)Dym/J mice to delete the Neo cassette. (J:152798)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ins2 Mutation: 93 strains or lines available

References

• Original: J:152798 Fan Y, et al., Thymus-specific deletion of insulin induces autoimmune diabetes. EMBO J. 2009 Sep 16;28(18):2812-24
• All: 4 reference(s)