Scly^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Scly^{tm1a(KOMP)Wtsi}
• Name: selenocysteine lyase; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362224
• Gene: Scly Location: Chr1:91226060-91248797 bp, + strand Genetic Position: Chr1, 46.08 cM
• Alliance: Scly^{tm1a(KOMP)Wtsi} page
• IMPC: Scly gene page

Mutation origin

• Mutant Cell Lines: EPD0044_6_C04, EPD0044_6_C05, EPD0044_6_D04, EPD0044_6_F04, EPD0044_6_F06, EPD0044_6_G04, EPD0044_6_G06, EPD0044_6_H04, EPD0044_6_H06
• Germline Transmission: Earliest citation of germline transmission: J:191114
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 91232539 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 91233396. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 2 lines available
• Carrying any Scly Mutation: 15 strains or lines available

Notes

Cell line EPD0044_6_G06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:191114

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 9 reference(s)