Elp1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Elp1^{tm1a(KOMP)Wtsi}
• Name: elongator complex protein 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362242
• Gene: Elp1 Location: Chr4:56749680-56802331 bp, - strand Genetic Position: Chr4, 31.66 cM
• Alliance: Elp1^{tm1a(KOMP)Wtsi} page
• IMPC: Elp1 gene page

Mutation origin

• Mutant Cell Lines: EPD0033_2_A09, EPD0033_2_A10, EPD0033_2_B09, EPD0033_2_B10, EPD0033_2_C09, EPD0033_2_C10, EPD0033_2_D09, EPD0033_2_D10, EPD0033_2_E10, EPD0033_2_F09, EPD0033_2_F10, EPD0033_2_G10, EPD0033_2_H09, EPD0033_2_H10
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_gt0 Mutation details: The L1L2_gt0 cassette was inserted at position 56796283 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 56796283. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Schematic for the Elp1^tm1a(KOMP)Wtsi and Elp1^tm1c(KOMP)Wtsi alleles

Expression

In Mice Carrying this Mutation:

14 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Elp1 Mutation: 69 strains or lines available

Notes

Cell line EPD0033_2_A09 was successfully used to make chimeric mice. Germline transmission was accomplished by the IKMC (project 25621). J:202989

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 11 reference(s)