Ap4e1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ap4e1^{tm1a(KOMP)Wtsi}
• Name: adaptor-related protein complex AP-4, epsilon 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362509
• Gene: Ap4e1 Location: Chr2:126850637-126909829 bp, + strand Genetic Position: Chr2, 61.76 cM
• Alliance: Ap4e1^{tm1a(KOMP)Wtsi} page
• IMPC: Ap4e1 gene page

Mutation origin

• Mutant Cell Lines: EPD0025_5_A07, EPD0025_5_A08, EPD0025_5_A09, EPD0025_5_B09, EPD0025_5_C07, EPD0025_5_C08, EPD0025_5_C09, EPD0025_5_E07, EPD0025_5_E09, EPD0025_5_F07, EPD0025_5_F09, EPD0025_5_G07, EPD0025_5_G09, EPD0025_5_H07, EPD0025_5_H08
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 126855606 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 126856446. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Ap4e1 Mutation: 59 strains or lines available

Notes

Cell line EPD0025_5_A08 was successfully used to make chimeric mice. Germline transmission was accomplished. J:200082

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 6 reference(s)