Strip1^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Strip1^{tm1a(KOMP)Wtsi}
• Name: striatin interacting protein 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362804
• Synonyms: Strip1^gt
• Gene: Strip1 Location: Chr3:107519848-107539010 bp, - strand Genetic Position: Chr3, 46.83 cM
• Alliance: Strip1^{tm1a(KOMP)Wtsi} page
• IMPC: Strip1 gene page

Mutation origin

• Mutant Cell Lines: EPD0316_1_A01, EPD0316_1_A02, EPD0316_1_B02, EPD0316_1_B03, EPD0316_1_C04, EPD0316_1_E04, EPD0316_1_F03, EPD0316_1_G01, EPD0316_1_G03, EPD0316_1_H03
• Germline Transmission: Earliest citation of germline transmission: J:251816
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 107531479 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 107532505. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 2 lines available
• Carrying any Strip1 Mutation: 42 strains or lines available

Notes

It is important to note that one Strip1 knockout-first cell line analyzed, EPD0316_1_A02, had a mixture of improperly targeted and correctly targeted ESC clones, based on Southern blotting, and was not used in J:251816. Cell line EPD0316_1_G03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:251816

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)