Ulk4^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ulk4^{tm1a(KOMP)Wtsi}
• Name: unc-51-like kinase 4; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362969
• Synonyms: Ulk4^-, Ulk4^tm1a
• Gene: Ulk4 Location: Chr9:120793520-121115225 bp, - strand Genetic Position: Chr9, 72.19 cM, cytoband F4
• Alliance: Ulk4^{tm1a(KOMP)Wtsi} page
• IMPC: Ulk4 gene page

Reduced body size and domed-shaped head of Ulk4^{tm1a(KOMP)Wtsi}/Ulk4^{tm1a(KOMP)Wtsi} (-/-) mice

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0182_4_B12, EPD0182_4_E10, EPD0182_4_E12, EPD0182_4_G12
• Germline Transmission: Earliest citation of germline transmission: J:211886
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 121088838 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 121089573. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Generation of the Ulk4^tm1a(KOMP)Wtsi allele

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Ulk4 Mutation: 83 strains or lines available

Notes

Special note about mutant cell line EPD0182_4_E12: The mutation generated in this ES cell line results in a hypomorphic allele. qRT-PCR analysis revealed a 78-96% reduction of mRNA expression in homozygous mutant mice (J:234631).

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 8 reference(s)