Cc2d1b^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cc2d1b^{tm1a(KOMP)Wtsi}
• Name: coiled-coil and C2 domain containing 1B; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362981
• Gene: Cc2d1b Location: Chr4:108477137-108491320 bp, + strand Genetic Position: Chr4, 50.64 cM
• Alliance: Cc2d1b^{tm1a(KOMP)Wtsi} page
• IMPC: Cc2d1b gene page

Mutation origin

• Mutant Cell Lines: EPD0016_1_A07, EPD0016_1_A08, EPD0016_1_A09, EPD0016_1_B07, EPD0016_1_B08, EPD0016_1_B09, EPD0016_1_C08, EPD0016_1_D08, EPD0016_1_E07, EPD0016_1_E08, EPD0016_1_F08, EPD0016_1_G07, EPD0016_1_H07, EPD0016_1_H08, EPD0016_1_H09, EPD0017_2_A11, EPD0017_2_C11, EPD0017_2_D11, EPD0017_2_D12, EPD0017_2_E11, EPD0017_2_E12, EPD0017_2_F12, EPD0017_2_G10, EPD0017_2_G11, EPD0017_2_G12, EPD0017_2_H11, EPD0017_2_H12
• Germline Transmission: Earliest citation of germline transmission: J:236761
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_st0
• Mutation details: The L1L2_st0 cassette was inserted at position 108480157 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 108481080. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Cc2d1b Mutation: 42 strains or lines available

Notes

Cell line EPD0017_2_C11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:236761

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 9 reference(s)