Gpt2^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gpt2^{tm1a(KOMP)Wtsi}
• Name: glutamic pyruvate transaminase (alanine aminotransferase) 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363039
• Gene: Gpt2 Location: Chr8:86219205-86254189 bp, + strand Genetic Position: Chr8, 41.61 cM, cytoband C2
• Alliance: Gpt2^{tm1a(KOMP)Wtsi} page
• IMPC: Gpt2 gene page

Reduction in brain size in Gpt2^{tm1a(KOMP)Wtsi}/Gpt2^{tm1a(KOMP)Wtsi} mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0041_2_B06, EPD0041_2_C04, EPD0041_2_F06, EPD0041_2_H06, EPD0067_1_A02, EPD0067_1_B01, EPD0067_1_B02, EPD0067_1_B03, EPD0067_1_C02, EPD0067_1_C03, EPD0067_1_D01, EPD0067_1_D02, EPD0067_1_E02, EPD0067_1_F02, EPD0067_1_G01, EPD0067_1_G03, EPD0067_1_H01, EPD0067_1_H02
• Germline Transmission: Earliest citation of germline transmission: J:238353
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_gt0 Mutation details: The L1L2_gt0 cassette was inserted at position 86231739 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 86232560. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:238353)

Generation of the Gpt2^tm1a(KOMP)Wtsi allele

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Gpt2 Mutation: 41 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 8 reference(s)