Smyd3^{tm2a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Smyd3^{tm2a(KOMP)Wtsi}
• Name: SET and MYND domain containing 3; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363150
• Gene: Smyd3 Location: Chr1:178779525-179345606 bp, - strand Genetic Position: Chr1, 83.48 cM, cytoband H3
• Alliance: Smyd3^{tm2a(KOMP)Wtsi} page
• IMPC: Smyd3 gene page

Mutation origin

• Mutant Cell Lines: EPD0013_1_A07, EPD0013_1_B07, EPD0013_1_C07, EPD0013_1_E07, EPD0013_1_G07, EPD0013_1_H07, EPD0022_2_A04, EPD0022_2_B04, EPD0022_2_B05, EPD0022_2_C04, EPD0022_2_C06, EPD0022_2_D04, EPD0022_2_D05, EPD0022_2_F04, EPD0022_2_F06, EPD0022_2_G04, EPD0022_2_G05, EPD0022_2_G06, EPD0022_2_H04, EPD0022_2_H05
• Germline Transmission: Earliest citation of germline transmission: J:213427
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 179250581 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 179251530. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smyd3 Mutation: 41 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)