Kmt5b^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kmt5b^{tm1a(KOMP)Wtsi}
• Name: lysine methyltransferase 5B; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363450
• Gene: Kmt5b Location: Chr19:3817421-3868303 bp, + strand Genetic Position: Chr19, 3.5 cM
• Alliance: Kmt5b^{tm1a(KOMP)Wtsi} page
• IMPC: Kmt5b gene page

Mutation origin

• Mutant Cell Lines: EPD0022_2_A02, EPD0022_2_A03, EPD0022_2_B03, EPD0022_2_C01, EPD0022_2_C02, EPD0022_2_C03, EPD0022_2_D01, EPD0022_2_D02, EPD0022_2_E02, EPD0022_2_E03, EPD0022_2_F01, EPD0022_2_F02, EPD0022_2_F03, EPD0022_2_G01, EPD0022_2_G02, EPD0022_2_G03, EPD0022_2_H01, EPD0022_2_H02
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 3842602 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 3843496. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Kmt5b Mutation: 43 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 13 reference(s)