Myo5a^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Myo5a^{tm1e(KOMP)Wtsi}
• Name: myosin VA; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363512
• Gene: Myo5a Location: Chr9:74978297-75130970 bp, + strand Genetic Position: Chr9, 42.26 cM
• Alliance: Myo5a^{tm1e(KOMP)Wtsi} page
• IMPC: Myo5a gene page

Diluted coat color in Myo5a^{tm1e(KOMP)Wtsi}/Myo5a^{tm1e(KOMP)Wtsi} mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0034_4_A04, EPD0034_4_A05, EPD0034_4_B03, EPD0034_4_B04, EPD0034_4_B05, EPD0034_4_C03, EPD0034_4_C04, EPD0034_4_C05, EPD0034_4_D03, EPD0034_4_D04, EPD0034_4_D05, EPD0034_4_E03, EPD0034_4_E04, EPD0034_4_E05, EPD0034_4_F03, EPD0034_4_F04, EPD0034_4_G03, EPD0034_4_G04, EPD0034_4_G05, EPD0034_4_H03, EPD0034_4_H04, EPD0034_4_H05
• Germline Transmission: Earliest citation of germline transmission: J:195694
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_gt0 Mutation details: The L1L2_gt0 cassette was inserted at position 75027487 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:195694)

Generation of the Myo5a^tm1e(KOMP)Wtsi allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 2 lines available
• Carrying any Myo5a Mutation: 265 strains or lines available

Notes

Cell line EPD0034_4_C03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:195694

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)