Cyp4b1^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyp4b1^{tm1e(KOMP)Wtsi}
• Name: cytochrome P450, family 4, subfamily b, polypeptide 1; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363698
• Gene: Cyp4b1 Location: Chr4:115481922-115504920 bp, - strand Genetic Position: Chr4, 53.06 cM
• Alliance: Cyp4b1^{tm1e(KOMP)Wtsi} page
• IMPC: Cyp4b1 gene page

Mutation origin

• Mutant Cell Lines: EPD0078_3_C02, EPD0078_3_E02, EPD0078_3_E03, EPD0078_3_F01, EPD0078_3_G01, EPD0078_3_G02, EPD0078_3_H02
• Germline Transmission: Earliest citation of germline transmission: J:207585
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 115499719 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:207585)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Cyp4b1 Mutation: 28 strains or lines available

Notes

Cell line EPD0078_3_G01 was successfully used to make chimeric mice. Germline transmission was accomplished. J:207585

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)