Tnnt3^{tm2a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tnnt3^{tm2a(KOMP)Wtsi}
• Name: troponin T3, skeletal, fast; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363809
• Synonyms: Tnnt3^lacZ
• Gene: Tnnt3 Location: Chr7:142052573-142069746 bp, + strand Genetic Position: Chr7, 87.94 cM
• Alliance: Tnnt3^{tm2a(KOMP)Wtsi} page
• IMPC: Tnnt3 gene page

Small size of the Tnnt3^{tm2a(KOMP)Wtsi}/Tnnt3⁺ mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0084_2_B02, EPD0084_2_C01, EPD0084_2_C02, EPD0084_2_C04, EPD0084_2_D04, EPD0084_2_G02, EPD0084_2_G03, EPD0084_2_G04, EPD0084_2_H04
• Germline Transmission: Earliest citation of germline transmission: J:204474
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 142063935 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 142064707. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:204474)

Generation of the Tnnt3^tm2a(KOMP)Wtsi allele

Expression

In Mice Carrying this Mutation:	30 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Tnnt3 Mutation: 23 strains or lines available

Notes

Cell line EPD0084_2_G03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:204474

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)