Ankle1^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ankle1^{tm1(KOMP)Wtsi}
• Name: ankyrin repeat and LEM domain containing 1; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363814
• Synonyms: Ankle1^-
• Gene: Ankle1 Location: Chr8:71858654-71862548 bp, + strand Genetic Position: Chr8, 34.43 cM
• Alliance: Ankle1^{tm1(KOMP)Wtsi} page
• IMPC: Ankle1 gene page

Mutation origin

• Mutant Cell Lines: EPD0323_2_A11, EPD0323_2_B10, EPD0323_2_B12, EPD0323_2_C10, EPD0323_2_E10, EPD0323_2_E11, EPD0323_2_F09, EPD0323_2_F11, EPD0323_2_G09, EPD0323_2_H10
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 3233 starting at position 71858819 and ending at position 71862052 of Chromosome 8 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Generation of the Ankle1^tm1(KOMP)Wtsi, Ankle1^tm1.1(KOMP)Wtsi and Ankle1^tm1.2(KOMP)Wtsi alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Ankle1 Mutation: 27 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 6 reference(s)