Ceacam16^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ceacam16^{tm1(KOMP)Wtsi}
• Name: CEA cell adhesion molecule 16; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363974
• Synonyms: Ceacam16^betagal
• Gene: Ceacam16 Location: Chr7:19586022-19595224 bp, - strand Genetic Position: Chr7, 9.95 cM
• Alliance: Ceacam16^{tm1(KOMP)Wtsi} page
• IMPC: Ceacam16 gene page

Mutation origin

• Mutant Cell Lines: EPD0319_6_B07, EPD0319_6_C08, EPD0319_6_E08
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 5464 starting at position 19587466 and ending at position 19592930 of Chromosome 7 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534, J:215775)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Ceacam16 Mutation: 24 strains or lines available

Notes

Cell line EPD0319_6_B07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:215775

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)