Fam13a^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Fam13a^{tm1e(KOMP)Wtsi}
• Name: family with sequence similarity 13, member A; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4364167
• Synonyms: Fam13a^-
• Gene: Fam13a Location: Chr6:58910521-59001487 bp, - strand Genetic Position: Chr6, 27.93 cM
• Alliance: Fam13a^{tm1e(KOMP)Wtsi} page
• IMPC: Fam13a gene page

Mutation origin

• Mutant Cell Lines: EPD0252_3_A06, EPD0252_3_A08, EPD0252_3_D07, EPD0252_3_F08, EPD0252_3_G05, EPD0252_3_H06
• Germline Transmission: Earliest citation of germline transmission: J:258290
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 58961360 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:258290)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 2 lines available
• Carrying any Fam13a Mutation: 45 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)