Sel1l^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sel1l^{tm1e(KOMP)Wtsi}
• Name: sel-1 suppressor of lin-12-like (C. elegans); targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4364219
• Gene: Sel1l Location: Chr12:91772817-91815931 bp, - strand Genetic Position: Chr12, 45.02 cM, cytoband E
• Alliance: Sel1l^{tm1e(KOMP)Wtsi} page
• IMPC: Sel1l gene page

Mutation origin

• Mutant Cell Lines: EPD0284_3_D08, EPD0284_3_F05
• Germline Transmission: Earliest citation of germline transmission: J:211773
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 91797973 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Sel1l Mutation: 34 strains or lines available

Notes

Conflicting reports exist for the integrity of the ES mutant cell line EPD0284_3_D08. A published report (J:206568) describes the line as carrying the Sel1l^{tm1a(KOMP)Wtsi} allele and the Sel1l^{tm1c(KOMP)Wtsi} allele derived from it. However, analysis at the Wellcome Trust Sanger Institute suggests that this ES cell line only carries the Sel1l^{tm1e(KOMP)Wtsi} allele.

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 5 reference(s)