Stac3^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Stac3^{tm1a(KOMP)Wtsi}
• Name: SH3 and cysteine rich domain 3; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4365152
• Gene: Stac3 Location: Chr10:127337555-127344692 bp, + strand Genetic Position: Chr10, 74.5 cM
• Alliance: Stac3^{tm1a(KOMP)Wtsi} page
• IMPC: Stac3 gene page

Abnormalities of Stac3^{tm1a(KOMP)Wtsi}/Stac3^{tm1a(KOMP)Wtsi} mice

Show the 7 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0101_1_A09, EPD0101_1_A10
• Germline Transmission: Earliest citation of germline transmission: J:200631
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 127338110 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 127340015. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Generation of the Stac3^tm1a(KOMP)Wtsi allele

Expression

In Mice Carrying this Mutation:	30 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 2 lines available
• Carrying any Stac3 Mutation: 30 strains or lines available

Notes

Cell lines EPD0101_1_A09 and EPD0101_1_A10 were successfully used to make chimeric mice. Germline transmission was accomplished. J:200631

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 7 reference(s)