Ern1^tm1Tiw
Targeted Allele Detail

Summary

• Symbol: Ern1^tm1Tiw
• Name: endoplasmic reticulum to nucleus signalling 1; targeted mutation 1, Takao Iwawaki
• MGI ID: MGI:4365396
• Synonyms: IRE1alpha^-
• Gene: Ern1 Location: Chr11:106285476-106378678 bp, - strand Genetic Position: Chr11, 69.14 cM, cytoband E1
• Alliance: Ern1^tm1Tiw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153222
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette replaced exons 11 - 17 via homologous recombination. Northern blot analysis confirmed the absence of RNA expression in homozygous MEFs. (J:153222)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ern1 Mutation: 58 strains or lines available

References

• Original: J:153222 Iwawaki T, et al., Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability. Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16657-62
• All: 7 reference(s)