Themis^tm1Hasu
Targeted Allele Detail

Summary

• Symbol: Themis^tm1Hasu
• Name: thymocyte selection associated; targeted mutation 1, Harumi Suzuki
• MGI ID: MGI:4365409
• Synonyms: Gasp^-
• Gene: Themis Location: Chr10:28544356-28759814 bp, + strand Genetic Position: Chr10, 16.06 cM
• Alliance: Themis^tm1Hasu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153235
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: The coding portion of exon 1 was replaced with a lacZ and floxed neo cassette. The absence of protein expression was confirmed by western blot analysis on thymocyte extracts. (J:153235)

Generation of the Themis^tm1Hasu allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Themis Mutation: 57 strains or lines available

References

• Original: J:153235 Patrick MS, et al., Gasp, a Grb2-associating protein, is critical for positive selection of thymocytes. Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16345-50
• All: 2 reference(s)