Mapk6^tm1Melo
Targeted Allele Detail

Summary

• Symbol: Mapk6^tm1Melo
• Name: mitogen-activated protein kinase 6; targeted mutation 1, Sylvain Meloche
• MGI ID: MGI:4365549
• Synonyms: Erk^-, Erk3^-, Mapk6^LacZNeo
• Gene: Mapk6 Location: Chr9:75294064-75317303 bp, - strand Genetic Position: Chr9, 42.3 cM, cytoband E1
• Alliance: Mapk6^tm1Melo page

Mapk6^tm1Melo/Mapk6^tm1Melo mice display cyanosis, carpoptosis, smaller body weight, and neonatal lethality

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153218
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2, 3, and 4 were replaced with a lacZ gene and neomycin resistance gene cassette inserted in-frame with the ATG start codon in exon 2. Quantitative RT-PCR and western blot analysis confirmed the absence of expression in lungs from homozygous mutants and homozygous MEFs, respectively. (J:153218)

Expression

In Mice Carrying this Mutation:	24 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mapk6 Mutation: 27 strains or lines available

References

• Original: J:153218 Klinger S, et al., Loss of Erk3 function in mice leads to intrauterine growth restriction, pulmonary immaturity, and neonatal lethality. Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16710-5
• All: 6 reference(s)