Rnf128^tm1.1Flv
Targeted Allele Detail

Summary

• Symbol: Rnf128^tm1.1Flv
• Name: ring finger protein 128; targeted mutation 1.1, Richard Flavell
• MGI ID: MGI:4365554
• Synonyms: GRAIL^-
• Gene: Rnf128 Location: ChrX:138464089-138573894 bp, + strand Genetic Position: ChrX, 61.35 cM
• Alliance: Rnf128^tm1.1Flv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153216
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP-flanked neomycin cassette was placed in intron 6 and a third loxP site was placed in intron 3. Cre mediated recombination removed exons 4 through 6. This deletion results in a out of frame mutation. The absence of protein expression was confirmed by western blot on spleen, thymus, kidney, liver, and brain extracts. (J:153216)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rnf128 Mutation: 10 strains or lines available

References

• Original: J:153216 Kriegel MA, et al., E3 ubiquitin ligase GRAIL controls primary T cell activation and oral tolerance. Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16770-5
• All: 2 reference(s)