Nkx3-1^{tm4(cre/ERT2)Mms}
Targeted Allele Detail

Summary

• Symbol: Nkx3-1^{tm4(cre/ERT2)Mms}
• Name: NK3 homeobox 1; targeted mutation 4, Michael M Shen
• MGI ID: MGI:4365630
• Synonyms: Nkx3-1^CreERT2
• Gene: Nkx3-1 Location: Chr14:69428141-69432107 bp, + strand Genetic Position: Chr14, 36.02 cM
• Alliance: Nkx3-1^{tm4(cre/ERT2)Mms} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153425
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Nkx3-1^{tm4(cre/ERT2)Mms} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nkx3-1 (MGI:97352)

• Mutation details: A cre/ERT2-polyA sequence was inserted at the translation start site of the Nkx3-1 gene, thus generating a null allele. The targeting vector also contained a self-excising ACE-cre/PolII-neo selection cassette for positive selection and a PGK-tk (thymidine kinase) cassette for negative selection. The vector was transfected into TC-1 ES cells which were used to generate chimeras. Excision of the selection cassette occurred by passage through the male germline. (J:153425)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nkx3-1 (mouse) Summary of all recombinase alleles driven by Nkx3-1. Comparative matrix view of recombinase activities Alliance mouse Nkx3-1 gene page

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Nkx3-1^{tm4(cre/ERT2)Mms}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 14 strains available Cell Lines: 0 lines available
• Carrying any Nkx3-1 Mutation: 38 strains or lines available

References

• Original: J:153425 Wang X, et al., A luminal epithelial stem cell that is a cell of origin for prostate cancer. Nature. 2009 Sep 24;461(7263):495-500
• All: 25 reference(s)