Lhx6^{tm1(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Lhx6^{tm1(cre/ERT2)Zjh}
• Name: LIM homeobox protein 6; targeted mutation 1, Z Josh Huang
• MGI ID: MGI:4365737
• Synonyms: Lhx6-CreER, Lhx6-CreERT2, Lhx6 delta neo
• Gene: Lhx6 Location: Chr2:35971965-35995420 bp, - strand Genetic Position: Chr2, 24.17 cM
• Alliance: Lhx6^{tm1(cre/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Lhx6^{tm1(cre/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lhx6 (MGI:1306803)

• Mutation details: A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the Lhx6 (LIM homeobox protein 6) gene. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. These Lhx6-CreERT2 mice were subsequently bred with wildtype siblings and/or wildtype C57BL/6 mice for at least one generation (and the FLPe transgene was removed) (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lhx6 (mouse) Summary of all recombinase alleles driven by Lhx6. Comparative matrix view of recombinase activities Alliance mouse Lhx6 gene page

Expression

In Mice Carrying this Mutation:

60 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lhx6 Mutation: 27 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 10 reference(s)