Tg(tetO/CMV-Prnp*,-lacZ)33Mjy
Transgene Detail

Summary

• Symbol: Tg(tetO/CMV-Prnp*,-lacZ)33Mjy
• Name: transgene insertion 33, Jiyan Ma
• MGI ID: MGI:4365814
• Synonyms: pBI-cyPrP-Gal, Tg(TRE/CMV-Prnp*,-lacZ)33Mjy
• Transgene: Tg(tetO/CMV-Prnp*,-lacZ)33Mjy Location: unknown
• Alliance: Tg(tetO/CMV-Prnp*,-lacZ)33Mjy page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: Transgenic mice express prion protein in the cytosol (cyPrnP, amino acids 23 to 230, which precisely eliminated the NH2-terminal and the COOH-terminal sequences that are cleaved upon ER entry), and beta-Gal under the regulation of a minimal CMV promoter and a tetracycline-responsive bi-directional promoter. The whole brain beta-Gal staining and Western blot revealed that beta-Gal and cyPrnp expression were largely confined to the forebrain. (J:80582, J:153494)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:153494 Wang X, et al., Cytoplasmic prion protein induces forebrain neurotoxicity. Biochim Biophys Acta. 2009 Jun;1792(6):555-63
• All: 2 reference(s)