Otx2^tm9Asim
Targeted Allele Detail

Summary

• Symbol: Otx2^tm9Asim
• Name: orthodenticle homeobox 2; targeted mutation 9, Antonio Simeone
• MGI ID: MGI:4365828
• Synonyms: Otx2^delta3SS
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^tm9Asim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153562
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The major and two cryptic splice sites upstream from the translation initiation codon were mutagenized and a floxed neo cassette was inserted into the downstream intron. The absence of protein expression of isoforms A and B was confirmed by western blot analysis. Expression of isoforms C and D are normal. (J:153562)

Expression

In Mice Carrying this Mutation:	54 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:153562 Acampora D, et al., Selective inactivation of Otx2 mRNA isoforms reveals isoform-specific requirement for visceral endoderm anteriorization and head morphogenesis and highlights cell diversity in the visceral endoderm. Mech Dev. 2009 Oct;126(10):882-97
• All: 1 reference(s)