Noto^{tm1(cre/ERT2)Hssk}
Targeted Allele Detail

Summary

• Symbol: Noto^{tm1(cre/ERT2)Hssk}
• Name: notochord homeobox; targeted mutation 1, Hiroshi Sasaki
• MGI ID: MGI:4366167
• Synonyms: Noto^{nmCherry-CreERT2}
• Gene: Noto Location: Chr6:85400868-85405859 bp, + strand Genetic Position: Chr6, 37.44 cM
• Alliance: Noto^{tm1(cre/ERT2)Hssk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153634
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Noto^{tm1(cre/ERT2)Hssk} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Noto (MGI:3053002)

• Mutation details: In this knock-in line, the coding sequences for nuclear fluorescent protein (Histone H2B-mCherry) and Cre/ERT2 were placed downstream of the Noto coding sequence, with sequences for virus-derived 2A peptides inserted between the protein coding sequences. (2A peptide-containing proteins are self-cleaved downstream of the 2A peptide, such that their introduction allows the production of multiple (3) proteins from a single transcript. The construct also included a neomycin resistance gene in a self-excising ACN cassette (cre recombinase driven by the murine angiotensin-converting enzyme lnked to a floxed neo cassette; the cassette is excised during spermatogenesis). The construct was electroporated into TT2 ES cells. Chimeric founders were crossed to C57BL/6 mice. Deletion of the ACN cassette was confirmed by PCR, (J:153634)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Noto (mouse) Summary of all recombinase alleles driven by Noto. Alliance mouse Noto gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Noto Mutation: 15 strains or lines available

References

• Original: J:153634 Ukita K, et al., Wnt signaling maintains the notochord fate for progenitor cells and supports the posterior extension of the notochord. Mech Dev. 2009 Oct;126(10):791-803
• All: 4 reference(s)