Bcl2l11^tm1.1Boui
Targeted Allele Detail

Summary

• Symbol: Bcl2l11^tm1.1Boui
• Name: BCL2 like 11; targeted mutation 1.1, Philippe Bouillet
• MGI ID: MGI:4366749
• Synonyms: Bim^B, Bim^Bad
• Gene: Bcl2l11 Location: Chr2:127967958-128004467 bp, + strand Genetic Position: Chr2, 62.23 cM, cytoband F3-G1
• Alliance: Bcl2l11^tm1.1Boui page

BH3 replacement mutations in Bcl2l11 prevent the development of polycystic kidney disease and spleen atrophy in Bcl2^tm1Dlo/Bcl2^tm1Dlo mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153846
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

Allele Type: Targeted Mutations: Insertion, Intragenic deletion Mutation details: The BH3 domain (in exon 5) was replaced by the mouse Bad BH3 domain by site-directed mutagenesis using PCR. A loxP-PGKneo-loxP cassette was inserted 840 bp downstream of exon 5. The neo cassette was removed by crossing the mice to cre deleter transgenic mice. (J:153846)

Generation of the Bcl2l11^tm1.1Boui allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bcl2l11 Mutation: 36 strains or lines available

References

• Original: J:153846 Merino D, et al., The role of BH3-only protein Bim extends beyond inhibiting Bcl-2-like prosurvival proteins. J Cell Biol. 2009 Aug 10;186(3):355-62
• All: 3 reference(s)