Gt(ROSA)26Sor^{tm1(CAG-taulacZ)Bene}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-taulacZ)Bene}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Robert Benezra
• MGI ID: MGI:4366911
• Synonyms: Gt(ROSA)26Sor^{tm1(CAG-MAPT/lacZ)Bene}, Gt(ROSA)26Sor^{tm1(CAG-taulacZ)Bene}, ROSA26^StLa, StLa, Stop-tauLacZ
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-taulacZ)Bene} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154023
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A genetic construct that reports cre recombinase activity with tauLacZ reporter gene was inserted in reverse orientation into the Gt(ROSA)26Sor safe harbor locus. The reporter construct included a CAG promoter, a loxP flanked stop cassette composed of neomycin resistance gene and multiple polyadenylation sequences, a tauLacZ reporter gene, and a polyadenylation sequence (pCAG-loxP-pEM7-Neo*-bGHpA-3xSV40pA-loxP-tauLacZ-bGHpA). The construct was electroporated into B6(Cg)-Tyrc-2J derived CY2.4 embryonic stem cells. Southern blot positive embryonic stem cell clones were injected into recipient C57BL/6J blastocysts. Positive male chimeras were bred with B6(Cg)-Tyrc-2J females. Albino pups were genotyped by PCR, then bred to B6J mice. (J:154023)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:154023 Nam HS, et al., High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell. 2009 Nov 6;5(5):515-26
• All: 6 reference(s)