Gt(ROSA)26Sor^tm1Afst
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1Afst
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, A Francis Stewart
• MGI ID: MGI:4367459
• Synonyms: Rosa26-rox-LacZ TG, Rosa-rox
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1Afst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154134
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to encode a sequence for the neomycin resistance gene with a polyadenylation signal (STOP sequence), flanked by rox sites (dre recombinase recognition sequences) and followed by the lacZ gene. This was targeted to the Gt(ROSA)26Sor locus in R1 ES cells. Following dre recombinase-mediated excision, beta-galactosidase expression can be detected in cells which underwent recombination. (J:154134)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:154134 Anastassiadis K, et al., Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Dis Model Mech. 2009 Sep-Oct;2(9-10):508-15
• All: 5 reference(s)