Chat/Slc18a3^tm1.1Vpra
Targeted Allele Detail

Summary

• Symbol: Chat/Slc18a3^tm1.1Vpra
• Name: choline O-acetyltransferase; targeted mutation 1.1, Vania Prado
• MGI ID: MGI:4367608
• Synonyms: VAChT^del
• Gene: Chat Location: Chr14:32130160-32187866 bp, - strand Genetic Position: Chr14, 19.4 cM
• Alliance: Chat/Slc18a3^tm1.1Vpra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153908
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intergenic deletion Mutation details: One loxP sequence was added 260 bp upstream from the translational initiation codon of Slc18a3 (within the first intron of Chat), and a second loxP was added approximately 1.5 kb downstream from the stop codon. This flanking region included the second exon of Chat. The TK-Neo cassette was added immediately after the second loxP and was followed by a third loxP (all in the same direction). The floxed region (including the TK-Neo cassette) was excised from mice by in vivo cre mediated recombination. No Slc18a3 mRNA was detected in homozygous mouse brain by qPCR. Slc18a3 protein was undetected in homozygous mutant mice by Western blot analysis of spinal cord extracts and immunostaining of the neuromuscular junction. Chat protein level was increased in E18.5 spinal cords and Chat mRNA level was increased as detected by qPCR from E18.5 mouse brains. (J:153908)

Generation of the Chat/Slc18a3^tm1Vpra, Chat/Slc18a3^tm1.1Vpra and Chat/Slc18a3^tm1.2Vpra alleles

Expression

In Mice Carrying this Mutation:	22 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chat Mutation: 58 strains or lines available

References

• Original: J:153908 de Castro BM, et al., The vesicular acetylcholine transporter is required for neuromuscular development and function. Mol Cell Biol. 2009 Oct;29(19):5238-50
• All: 4 reference(s)