Ilk^tm8.1Ref
Targeted Allele Detail

Summary

• Symbol: Ilk^tm8.1Ref
• Name: integrin linked kinase; targeted mutation 8.1, Reinhard Fassler
• MGI ID: MGI:4368012
• Synonyms: Ilk^K220A
• Gene: Ilk Location: Chr7:105385799-105392132 bp, + strand Genetic Position: Chr7, 55.97 cM, cytoband E1
• Alliance: Ilk^tm8.1Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154054
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Lysine codon 220 (AAG) in exon 8 was changed to alanine (p.K220A) and a loxP site flanked neomycin resistance gene cassette was inserted into the 3' UTR in exon 13. The neo cassette was removed through subsequent Cre-mediated recombination. This substitution produces a kinase-dead product with decreased binding to beta-parvin (Parvb). (J:154054)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ilk Mutation: 18 strains or lines available

References

• Original: J:154054 Lange A, et al., Integrin-linked kinase is an adaptor with essential functions during mouse development. Nature. 2009 Oct 15;461(7266):1002-6
• All: 2 reference(s)