Rab18^{Gt(EUCE0233a03)Hmgu}
Gene trapped Allele Detail

Summary

• Symbol: Rab18^{Gt(EUCE0233a03)Hmgu}
• Name: RAB18, member RAS oncogene family; gene trap EUCE0233a03, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4369232
• Gene: Rab18 Location: Chr18:6765167-6791606 bp, + strand Genetic Position: Chr18, 4.53 cM
• Alliance: Rab18^{Gt(EUCE0233a03)Hmgu} page

Mutation origin

• Mutant Cell Line: EUCE0233a03
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: rsFlipROSAbgeo*
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. The gene trap vector inserted into intron 2. The genetrap allele initiates transcription from the endogenous promoter and prematurely terminates at the polyadenylation sequence in the FlipROSABetaGeo vector, resulting in a truncated mRNA that encodes a non-functional protein. RT-PCR of E11.5 embryos using primers flanking the gene trap insertion indicates very weak mRNA expression. However, RT-PCR using primers further towards the 3'-end (exons 3-7) indicates alternate transcripts. Quantitative real-time PCR that amplifies exons 4-5 shows that alternate transcripts are present at very low levels. Western blot analysis of sciatic nerves and mouse embryonic fibroblasts, however, shows no protein expression or expression of any smaller protein isoforms, indicating a null allele. (J:211808)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  EUCE0233a03.p1ka3SPK	FI527619 ; (MGI Seq Detail)	3'Splinkerette PCR	Chr18:6777946-6778670 bp (+)
  EUCE0233a03.q1k5SPK	FI540905 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr18:6777749-6777920 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  20 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rab18 Mutation: 24 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)