Khk^tm2Dtb
Targeted Allele Detail

Summary

• Symbol: Khk^tm2Dtb
• Name: ketohexokinase; targeted mutation 2, David T Bonthron
• MGI ID: MGI:4398729
• Synonyms: KHK-A/C KO, Khk^delta
• Gene: Khk Location: Chr5:31078780-31088592 bp, + strand Genetic Position: Chr5, 16.9 cM
• Alliance: Khk^tm2Dtb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154144
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A stop codon was introduced into exon 3a preventing the production of the A transcript. A floxed neo cassette was inserted upstream of the modified exon 3a. Exons 4 through 6 and part of 7 were deleted, preventing the production of the C splice variant. Western blot analysis confirmed the absence of isoforms A and C. (J:154144, J:166959)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Khk Mutation: 24 strains or lines available

References

• Original: J:154144 Diggle CP, et al., Ketohexokinase: expression and localization of the principal fructose-metabolizing enzyme. J Histochem Cytochem. 2009 Aug;57(8):763-74
• All: 28 reference(s)