Spdef^tm1.1Cle
Targeted Allele Detail

Summary

• Symbol: Spdef^tm1.1Cle
• Name: SAM pointed domain containing ets transcription factor; targeted mutation 1.1, Hans Clevers
• MGI ID: MGI:4398888
• Gene: Spdef Location: Chr17:27933326-27947929 bp, - strand Genetic Position: Chr17, 14.59 cM, cytoband B1
• Alliance: Spdef^tm1.1Cle page

Absence of mucous (goblet) cells in tracheal and laryngeal submucosal glands in Spdef^tm1.1Cle/Spdef^tm1.1Cle mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154852
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An frt flanked neo cassette with a 3' loxP site was inserted upstream of exon 6 and an additional loxP site was inserted downstream of exon 6. Germ line, cre mediated recombination removed exon 6 leaving the frt flanked neo cassette intact. (J:154852)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spdef Mutation: 15 strains or lines available

References

• Original: J:154852 Gregorieff A, et al., The ets-domain transcription factor Spdef promotes maturation of goblet and paneth cells in the intestinal epithelium. Gastroenterology. 2009 Oct;137(4):1333-45.e1-3
• All: 4 reference(s)