Zap70^tm2.1Weis
Targeted Allele Detail

Summary

• Symbol: Zap70^tm2.1Weis
• Name: zeta-chain (TCR) associated protein kinase; targeted mutation 2.1, Arthur Weiss
• MGI ID: MGI:4399123
• Synonyms: YYAA
• Gene: Zap70 Location: Chr1:36800912-36821899 bp, + strand Genetic Position: Chr1, 15.41 cM, cytoband A4-C1
• Alliance: Zap70^tm2.1Weis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154166
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted Mutation: Nucleotide substitutions Mutation details: Residues Y315 and Y319 present in exon 7 of ZAP-70 were mutated to alanine. A diagnostic EcoRV site was also introduced into exon 7 as well as a floxed neo cassette in intron 2. Cre mediated recombination in the male germline subsequently removed the neo cassette. (J:154166)

Generation of the Zap70^tm2.1Weis allele

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zap70 Mutation: 55 strains or lines available

Notes

ES cells used contained Tg(Prm-cre)70Og

References

• Original: J:154166 Hsu LY, et al., A hypomorphic allele of ZAP-70 reveals a distinct thymic threshold for autoimmune disease versus autoimmune reactivity. J Exp Med. 2009 Oct 26;206(11):2527-41
• All: 2 reference(s)