Wnt8a^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Wnt8a^{tm1(KOMP)Vlcg}
• Name: wingless-type MMTV integration site family, member 8A; targeted mutation 1, Velocigene
• MGI ID: MGI:4399164
• Gene: Wnt8a Location: Chr18:34675380-34681114 bp, + strand Genetic Position: Chr18, 18.69 cM, cytoband B3
• Alliance: Wnt8a^{tm1(KOMP)Vlcg} page
• IMPC: Wnt8a gene page

Mutation origin

• Mutant Cell Lines: 10481B-F10, 10481B-H3
• Germline Transmission: Earliest citation of germline transmission: J:194132
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 5486bp between positions 34675407-34680892 of Chromosome 18 (Build GRCm39). The entire coding sequence was replaced with a lacZ gene and floxed neo cassette. Whole mount RNA hybridization confirmed the absence of transcript expression in E8 embryos. (J:136110, J:194132)

Expression

In Mice Carrying this Mutation:

27 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Wnt8a Mutation: 15 strains or lines available

Notes

Cell line 10481B-F10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:194132

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)