Actl7b^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Actl7b^{tm1(KOMP)Vlcg}
• Name: actin-like 7b; targeted mutation 1, Velocigene
• MGI ID: MGI:4399183
• Synonyms: Actl7b^-
• Gene: Actl7b Location: Chr4:56740005-56741425 bp, - strand Genetic Position: Chr4, 31.66 cM
• Alliance: Actl7b^{tm1(KOMP)Vlcg} page
• IMPC: Actl7b gene page

Mutation origin

• Mutant Cell Line: 10808A-C2
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 1321bp between positions 56740033-56741353 of Chromosome 4 (Build GRCm39). IHC and Western blot analysis confirmed the absence of protein expression in the testes of adult homozygous mutant mice. (J:136110, J:334022)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Actl7b Mutation: 18 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)